146种常用培养基配方(编辑修改稿)内容摘要:

Yeast Sporulation Medium (酵母菌产生子囊孢子培养基 ) (1) Kleyn Medium KH2PO4 % K2HPO4 % Sodium acetate ( 醋酸钠 ) % Glucose (葡萄糖 ) % NaCl % Peptone (蛋白胨 ) % Biotin (生物素 ) 2μg% Agar ( 琼脂 ) 2% Trace element solution (混合盐类 ) 1ml/100ml [Note]: Trace element solution (混合盐类溶液): %, NaCl %, %, %, % (2) Gorodkowa Medium Glucose (葡萄糖 ) % Peptone (蛋白胨 ) 1% NaCl % Agar (琼脂 ) 2% (3) McClary Medium Glucose (葡萄糖 ) % KCl % Yeast juice (酵母汁 ) % Sodium actate (醋酸钠 ) % Agar (琼脂 ) % 32. BPY Medium (BPY 培养基 ) Beef extract (牛肉膏 ) 5g Prptone (蛋白胨 ) 10g NaCl 5g Yeast extract (酵母膏 ) 5g Glucose (葡萄糖 ) 5g Agar (琼脂 ) 12% Distilled (蒸馏水 ) 1000ml pH 适用范围:大肠埃希氏菌(大肠杆菌)、恶臭假单胞菌 33. LB Medium (LB 培养基 ) Yeast extact (酵母膏 ) 5g Peptone (蛋白胨 ) 10g NaCl 10g Agar (琼脂 ) 12% Distilled water (蒸馏水 ) 1000ml pH 适用范围:大肠埃希氏菌(大肠杆菌) 34. Penssay Broth (Penssay 肉汤 ) Beef extract (牛肉膏 ) Yeast extract (酵母 膏 ) Tryptone (胰蛋白胨 ) 5g NaCl Glucose (葡萄糖 ) 1g Distilled water (蒸馏水 ) 1000ml pH 35. Blond Agar (血琼脂培养基 ) Peptone (蛋白胨 ) 10g Glucose (葡萄糖 ) 2g NaCl 5g NaH2PO4 Sodium citrate (柠檬酸钠 ) 5g Brain infusion (脑浸汁 ) 800ml Pig heart infusion (猪心浸 ) 200ml Sleep Blood (羊血 ) 10% pH [Note]: Preparation of pig brain and pig heart infusion: ( 1) Smash up 100g fresh pig heart ( without fat and blood vessel) .Add distilled water up to 1000ml。 ( 2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml。 ( 3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes。 ( 4) Cool and filtrate through gauze . [注 ]猪心和猪脑浸汁法:( 1)新鲜猪心除去心头和脂肪,搅碎 100克加蒸馏水至 1000 毫升;( 2)新鲜猪脑除去血管,取 200克加蒸馏水至 1000毫升;( 3) 50℃ 保温 1 小时,然后煮开 15 分钟;( 4)冷却后用纱布过 滤备用 36. Cellulose ﹣ deposing Bacteria Synthetic Medium (纤维细菌合成培养基 ) NaCl 6g KH2PO4 CaCl2 K2HPO4 Yeast extract (酵母膏 ) (NH4)2SO4 Distilled water (蒸馏水 ) 1000ml Filter paper strip: one strip (滤纸条:一片 ) pH [Note]: So。 id medium for cellulosedeposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (斜面培养基去滤纸条用 2%琼脂及 2%羧甲基纤维素钠 CMCNa ) 37. Clostridium Pasreurianum Synthetic Medium (巴氏梭状芽孢杆菌合成培养基) Glucose (葡萄糖) 1% MnSO4 % KH2PO4 % FeSO4 % K2HPO4 % Yeast extract(酵母膏) % % Peptone (蛋白胨 ) % NaCl % CaCO3 % Distille water (蒸馏水 ) 100% pH 38. Yeast ExtractMedium Extract Agar Ⅱ (酵母膏、麦芽膏琼脂 Ⅱ ) Yeast extract (酵母膏) 4g Mall extract (麦芽膏) 10g Glucose (葡萄糖) 4g Agar (琼脂) 17g Distilled water (蒸馏) 1000ml pH 39. Oatemeal Agar (燕麦粉琼脂) Oatemeal( infusion) [燕麦粉浸液 ] 20g Agar (琼脂) 18% Trace element solution (迹量盐溶液) 1ml Distilled water up to (用蒸馏水补充至 1000 毫升) 1000ml pH [Note]: Trace element solution (迹量盐溶液 ): Distilled water (蒸馏水 ) 100ml 40. Calcium Malate Agar (苹果酸钙琼脂 ) Calium malate ( 苹果酸钙 ) 10g K2HPO4 NH4Cl Glycerol (甘油 ) 10g Agar (琼脂 ) 15g Distilled water (蒸馏水 ) 1000ml 41. Thiobacillus Ferrooxidans Medium (氧化亚铁硫杆菌培养基 ) (NH4)2SO4 KH2PO4 KCl Ca(NO3) 50g Distilled water (蒸馏水 ) 1000ml pH 42. Thiobacillus Thiooxidans Medium (氧化硫硫杆菌培养基 ) (NH4)2SO4 KH2PO4 34g CaCl2 (anhydrous) [ 无水 ] Distilled water (蒸馏水 ) 1000ml pH 43. Plasma Substitute Medium (代血浆培养基 ) Sucrose (蔗糖 ) % Peptone (蛋白胨 ) % KH2PO4 % % pH 44. Skim Milk Medium (脱脂牛奶培养基 ) ATCC 183 脱脂牛奶 100g 去离子水 1000ml 适用范围:保加利亚乳杆 菌、嗜热乳酸链球菌 45. Pea Agar (豌豆琼脂 ) Glucose (葡萄糖 ) 1% Peptone (蛋白胨 ) % NaCl % Agar (琼脂 ) % Pea infusion (counted by NH2) [豌豆浸汁 (以 NH2 计 )] 1214 mg∕100 pH (before sterilization) [消前 ] [Note]: Preparetion of pea infusion (豌豆浸汁的制法 ) : Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 710 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (称取白豌豆 900克,置于 5 公升广口瓶中,加自来水 3公升,用硫酸调 pH 至 ,再加氯仿 30 毫升(防腐),加胶塞并用纱布扎口(防止冲脱),放 37℃ 恒温 室710 天。 取出用滤纸过滤,滤液煮沸20 分钟,以除氯仿,并再次以滤纸过滤,除沉淀。 滤液分装,于 120℃ 30分钟灭菌。 后冷藏备用。 ) The standard quality of pea infusion as following (豌豆浸汁质量标准 ): Aminonitrogen(氨基酸) : 160200 mg∕100ml Soluble phosphoruse (溶磷) : 400600 μg∕ml Color(色泽) : auratus,transparent (金黄、透明) 46. Wheat Bran Medium (麦麸琼脂 ) Wheat bran (麦麸 ) % (NH4)2HPO4 % K2HPO4 % % Agar (琼脂 ) 2% pH 适用范围:金霉素链霉菌、红霉素链霉菌 47. Glucose Yeast Extract Agar (葡萄糖、酵母膏琼脂 ) Glucose (葡萄糖 ) 10g Yeast extract (酵母膏 ) 10g Water (水 ) 1000ml Agar (琼脂 ) 15g pH 48. MMN Medium (MMN 培养基 ) CaCl2 MgSO4 NaCl FeCl3 (1%) KH2PO4 Vitamib B1 (硫胺素 ) 100μg (NH4)2HPO4 Wort (12 Be′) 100ml Glucose (葡萄糖 ) 1015g Citric acid (柠檬酸 ) Distilled water (蒸馏水 ) 900ml pH (左右 ) 适用范围:蜜环菌 49. Starch Agar (淀粉琼脂 ) Soluble starch (可溶性淀粉 ) 10g NaNO3 1g MgCO3 1g K2HPO4 NaCl Agar (琼脂 ) 20g Distilled water (蒸馏水 ) 1000ml 50. 5 Be′Wort。
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