fda三聚氰胺(melamine)检测标准(编辑修改稿)

fda三聚氰胺(melamine)检测标准(编辑修改稿)内容摘要：

eam of dry air using a ReactiVap module set at approximately 70176。 C. Add 200 181。 l pyridine and 200 181。 l BSTFA, vortex briefly to mix, and incubate at approximately 70176。 C for 30 minutes. Known Samples: In general, if working with a known concentration of analyte (. standards), appropriate dilutions should be made with pyridine/BSTFA to achieve an on column concentration no greater than 100 181。 g/mL. After calculating suitable dilutions, transfer an appropriate amount of the standard solution to an autosampler vial. Evaporate to dryness under a stream of dry air using a ReactiVap module set at approximately 70176。 C. Add 200 181。 l pyridine and 200 181。 l BSTFA, vortex briefly to mix, and incubate at approximately 70176。 C for 30 minutes. Unknown Samples: Transfer 40 181。 l of the methanol extract prepared for analysis in section A into an autosampler vial. Evaporate to dryness under a stream of dry air using a ReactiVap module set at approximately 70176。 C. Add 200 181。 l pyridine and 200 181。 l BSTFA, vortex briefly to mix, and incubate at approximately 70176。 C for 30 minutes. C. Sample Fortification: This method was successfully applied to detect melamine in wet and dry pet food posites (Figure 2) and dry wheat glutens (Figure 3). Based on HPLCUV results, this method was able to detect melamine levels down to % (by weight) in official FDA samples using ~ g samples with TMS derivatization and splitless injection. Uncontaminated wheat gluten and pet food posites were spiked w。