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27282  Food worker behavior – Observational study (FDA JIFSAN UMD collaboration) – Lubran MB, et al. (2020) J Food Protection, 73 (10):184957. 17 May 22nd, 2020 Interagency Risk AssessmentListeria monocytogenes in Retail Delicatessens Public Meeting Lubran MB, et al. (2020) J Food Protection, 73 (10):184957 Example 1: Which Sites? Which Events? Observational study in retail environment Action No. Action Sequence 1. 10am Wash hands 2. Put on gloves 3. Open case 4. Pick up salami 5. Close case 6. Put salami on slicer 7. Slice salami on gloves 8. Put salami on tissue 9. Touch scale 10. Give bag to customer Process Objects Involved Bacteria inactivation hands Cross contamination Case glove Cross contamination Case glove Cross contamination Partitioning Cross contamination Chub – product sold 1st slice – glove Cross contamination Scale glove 18 May 22nd, 2020 Interagency Risk AssessmentListeria monocytogenes in Retail Delicatessens Public Meeting Additional Data/Model • Growth model – Mejholm and Dalgaard, 2020 J Food Prot, 72(10), 21322143. • Temperature in deli case – Ecosure 2020 data ( .) • Time and temperature during transport and at home – Ecosure 2020 data ( .) • Consumption data – NHANES study, WWEIA data, 19992020 • Dose response model – FAO/WHO 2020 19 0 5 10 2 4 6 8 time (weeks) 0 5 10 15 20 0 2 4 6 8 10 time (days) 0 2 4 6 8 10 0 20 40 60 80 0 10 20 30 40 0 2 4 6 8 10 log 10 ufc.ml 1 log 10 ufc.ml 1 log 10 ufl 1 (e) (g) (h) (i) time (days) time (days) 176。 C 176。 C 0 20 40 60 80 2 0 2 4 6 (f) time (weeks) May 22nd, 2020 Interagency Risk AssessmentListeria monocytogenes in Retail Delicatessens Public Meeting Outline • Design • Data Sources • Implementation • Modeling Approach • Verification 20 May 22nd, 2020 Interagency Risk AssessmentListeria monocytogenes in Retail Delicatessens Public Meeting R model “Blue Meadow” cluster 2,016 cores, 21 TFlops Available through the Office of Science and Engineering Laboratories, CDRH FDA Parallel puting Parameters Excel Data File A Technical Challenge… • Discrete Event model + Few bacteria in the system = Slow convergence • Each simulation: 100 Stores。 1,000,000 Servings • Currently: 126 Scenarios T r a n s fe r s : lo g 1 0 (T 0 .B a s e lin e + 1 )ToFromMe atC he es eS ala dH an dG lo veF lo orS in kHa nd leS ca leC as eUten silU te ns ilH an dleS lic erF CSNF CSS oldN ic heR aw Fo odGrow thMe atC he es eS ala d。