生物医学工程外文翻译内容摘要:
ts have been designed with quartz glass to have good transmission in UV region at 340 nm. Keeping in view the low response of photo detector in UV (340 nm), all the optical ponents have been provided with enhanced antireflection coating in the UV region. In the optomechanical assembly, special care has been taken in the design so that each ponent is properly aligned with respect to optical axis. To get the required wavelength of light to be passed, 6 interference filters of different wavelengths such as 340 nm, 405 nm, 505 nm, 546 nm, 578 nm, and 630 nm, from UV region to visible region spectrum (300nm to 700 nm), have been mounted on the filter wheel. These filters are selected automatically depending on the test performed. When the filter of required wavelength is selected, the corresponding gain is selected automatically. The filter wheel is driven by a stepper motor, which is interfaced with the port of microcontroller through driver circuit. Pulses are generated according to required sequence to rotate the motor at required angle, which brings the filter in front of photo detector. The diagram of filter wheel assembly is shown in Figure 5. 咸宁学院本科毕业设计(论文): 全自动生化分析仪常见故障诊断、分析与维修 16 . Methods (i) Signal processing Advances in electronics and microcontroller technology have played a central role in signal processing [5]. Computers are included in automated analysis for data acquisition and processing of analytical data. Output of photodiode and preamplifier is a voltage which varies directly with the light which is passed through the flow cell and selected wavelength filter. Preamplifier gain is selected automatically as per the selected test parameter and filter. Output of preamplifier is converted into digital value by a 12bit analog to digital converter. Microcontroller performs calculations on these digital values according to the appropriate calculation algorithms developed [6]. . Methods for calculation The instrument developed works in four different modes such as concentration (end point), kiic mode, fixedtime mode, and absorbance mode. For measurement of concentration in different modes, different formulas are used as shown below. (1) Concentration (end point)mode: Concentration of sample=Abs. Sample(Conc. of Standard/Abs. of Standard) (6) Or Concentration of sample = Abs. Sample F. (7) (2) Kiic mode: Concentration (U/L)=ΔAbs ./ Min( Conc. of Standard/Abs/min of Standard) (8) Or =ΔAbs / MinF. (3) Fixedtime mode: Concentration (U/L)=ΔAbs( Conc. of Standard/Abs/min of Standard) (9) Or =ΔAbsF. 咸宁学院本科毕业设计(论文): 全自动生化分析仪常见故障诊断、分析与维修 17 Figure 6: Flow diagram of system software. Where F = . 106/(. Absorptivity P) (10) . is total reaction volume, . is sample volume, P is path length in cm, Abs. is absorbance, Conc. is concentration, and Min. is minute. . System software The layout of the steps followed in the development of the software of the instrument has been provided in the flow chart shown in Figures 6 and 7. On the basis of this flow diagram, system software has been developed using “C” cross piler [7, 8] for Intel 8031 microcontroller in modular form. System software is menu driven and user friendly. Many advance features have been incorporated in the software for fast and robust operation. The main program calls the subfunctions and executes them accordingly. Systemsoftware is stored in the system EPROM. 3. RESULTS AND DISCUSSION In this system, the programming, reading, and reporting operations are easy and user friendly. The 咸宁学院本科毕业设计(论文): 全自动生化分析仪常见故障诊断、分析与维修 18 instrument is provided with a keyboard which facilitates quick change from one function to another and setting of parameters which can be monitored on LCD with both alphanumeric as well as graphics capabilities without going through plex sequential operations. Printer in the system is used for test reports of the patients. The system was clinically evaluated successfully over 1000 blood samples at the Department of Biochemistry, GovernmentMedical College amp。 Hospital, Sector32, Chandigarh. 17 blood parameters have been analysed and results have been found to be satisfactory. Figure 7: Flow diagram of system software. 4. CONCLUSION The instrument developed is universally useful for small clinical laboratories, big hospitals, and nursing homes for qualitative analysis of blood. The instrument is capable of handling a reasonable amount of workload. The work anization。生物医学工程外文翻译
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