titlepage长庚大学生物医学研究所生理暨药理组博士论文探

titlepage长庚大学生物医学研究所生理暨药理组博士论文探内容摘要：

viations (縮寫表 )…………………………………………………………… .v Pharmacological inhibitors (藥理性抑制劑 )…………………………………… ...vii Abstract in Chinese (中文摘要 )………………………………………………… ...viii Abstract in English (英文摘要 )…………………………………………………… xii Table of Contents (目錄 )………………………………………………………… ...xvi CHAPTER I INTRODUCTION…………………………………………… .....1 SECTION 1. Background and significance…………………………………… ....2 SECTION 2. Specific aims……………… ……………………………………… 18 SECTION 3. Figures and tables……………………………………………… .....19 CHAPTER II MATERIALS AND METHODS…………………………… ....57 SECTION 1. Materials………………………………………………………… ...58 SECTION 2. Methods………………………………………………………… ....60 Table of Contents (2) CHAPTER III (Results) ………………………………………………………… ...69 Induction of cytosolic phospholipase A2 by lipopolysaccharide in canine tracheal smooth muscle cells: Involvement of MAPKs and NFκB pathways SECTION 1: Background…………………………………………………………… 70 SECTION 2: Results……………………………………………………………… ....73 SECTION 3: Discussion…………………………………………………………… ..79 SECTION 4: Figures and legends…………………………………………………… 83 CHAPTER IV (Results)…… .…………………………………………………… ..100 Involvement of MAPKs and NFκB in LPSinduced VCAM1 expression in human tracheal smooth muscle cells CHAPTER V (Results) ..… ..…………………………………………………… ...127 Lipopolysaccharide induces VCAM1 expression and neutrophil adhesion to human tracheal smooth muscle cells: Involvement of Src/EGFR/PI3K/Akt pathway CHAPTER VI (Results)………………………………………………………… ..157 Lipopolysaccharide upregulates VCAM1 expression in human tracheal smooth muscle cells via PKC/MAPKs/AP1 activation CHAPTER VII CONCLUSION AND PERSPECTIVES..................................188 SECTION 1: Conclusion............................................................................................189 SECTION 2: Perspectives..........................................................................................192 SECTION 3: Schemes……………………………………………………………… 193 PUBLICATIONS………………………………………………………………… ..194 REFERENCES ........................................................................................................196 SECTION 1. Background and significance Roles of proinflammatory protein expression in airway inflammatory diseases The importance of airway smooth muscle cells in airway inflammatory diseasess Role of lipopolysaccharide in airway inflammatory diseasess Role of cytosolic phospholipase A2 in airway inflammatory diseases Role of adhesion molecules in airway inflammatory diseases CHAPTER I INTRODUCTION Roles of MAPK pathways in airway inflammatory diseases Role of PI3kinase/Akt pathways in airway inflammatory disease Role of Calcium in airway inflammatory diseases Role of PKC in airway inflammatory diseases Role of RTKs in airway inflammatory disease Role of NFB and AP1 in airway inflammatory diseases Role of p300 in airway inflammatory diseases The intracellular signalings of Tolllike receptors (TLRs) SECTION 2. Specific aims The overall objective of this work is to establish the mechanisms of LPS action on the TSMCs that regulates the expression of VCAM1 thus results in enhancement of cell adhesion and inflammation. Our hypothesis of this proposal is that upregulation of cPLA2 and VCAM1 induced by LPS may contribute to leukocyte/TSMCs interaction and exaggerate inflammatory responses in airways. To evaluate the hypothesis, the specific aims addressed are: 1. To investigate the protein and mRNA expression of cPLA2 and VCAM1 induced by LPS in TSMCs. 2. To。