基因工程课程简介

基因工程课程简介内容摘要：

Transformation and transfection Packaging phage DNA in vitro Alternative DNA delivery methods 本章要点： 掌握基因工程受提细胞的类型和要求。 掌握载体的基本结构和质粒载体、噬菌体载体的特点、构建原理。 掌握在体外源 DNA 连接的方式和原理 以及感受态菌的制备原理。 掌握基因转入受体细胞的基本方法和原理。 （重点） Chapter 6 Cloning strategies (6hrs) which approach is best Cloning from mRNA Synthesis of cDNA Cloning cDNA in plasmid vectors Cloning cDNA in bacteriophage vectors Cloning from genomic DNA Genomic libraries Preparation of DNA fragments for cloning Ligation, packaging and amplification libraries Advanced cloning strategies Synthesis and cloning of cDNA Expression of cloned cDNA molecules Cloning large DNA fragments BAC and YAC vectors 本章要点：掌握化学合成 DNA的原理和 DNA 文库的构建原理。 了解酵母人 工染色体和细菌人工染色体的结构和工作原理。 （ 次 重点） Chapter 7 The polymerase Chain reaction (4hrs) The history of PCR The methodology of the PCR The essential features of the PCR The design of primers for PCR DNA polymerases for PCR More exotic PCR techniques PCR using mRNA templates Nested PCR Inverse PCR RAPD and several other acronyms Processing of PCR products Application of the PCR 本章要点：掌握 PCR 扩增 DNA 的技术原理和方法。 （重点） Chapter 8 Selection, Screening and analysis of rebinants (6hrs) Geic selection and screening methods The use of chromogenic substrates Insertional inactivation Complementation of defined mutations Other geic selection methods Screening using nucleic acid hybridisation Nucleic acid and probes Screening clone banks Immunological screening for expressed genes Analysis of cloned genes Characterisation based on mRNA translation in vitro Restriction mappin Blotting techniques DNA sequencing 本章要点：掌握常用的抗菌素抗性筛选、酶切筛选、 杂交筛选、 酶联免疫吸附测定（ ELISA）、免疫印迹（ western blotting）法的原理。 了解转译筛选法和真核生物的报道基因筛选法。 （重点） Part III Geic Engineering in Action (12hrs) Chapter 9 Understanding genes and genomes (self study) Analysis of gene structure and function A closer look at sequences Finding important。