苏教版生物选修1第二节植物组织培养技术

苏教版生物选修1第二节植物组织培养技术内容摘要：

loped over the surface of the explant, along with roots from the lower surface. The result is parable with the control medium 二、相关知识 No BAP BAP reduced to mg. l1 No NAA Poor shoot development and no roots. Extensive callus generation Poor shoot development and no roots NAA reduced to mg. l1 More balanced development of roots and shoots. However, undifferentiated callus is developing at the edges of the explant Profuse development of roots and a reduced number of shoots. Undifferentiated callus is developing at the edges of the explant 二、相关知识 NAA increased to mg. l1 Profuse development of roots over the explant upper and lower surface, and few shoots. Some callus No MS salts No sign of regeneration from explant at all. MS salts reduced to g. l1 Some root growth but reduced development of shoots MS salts increased to g. l1 Shoots developed on upper surface of explant. No roots or callus. 二、相关知识 植物细胞的全能性 植物细胞具有该植物体全部遗传的可能性，在一定条件下具有发育成完整植物体的潜在能力。 差异 :(1) 受精卵的全能性最高 (2) 受精卵分化后的细胞中，体细胞的全能性比生殖细胞的低。 潜在全能性的原因 ： 基因表达的选择性 科学研究表明，处于离体状态的植物活细胞，在一定的营养物质、激素和其他外界条件的作用下，就可能表现出全能性，发育成完整的植株。 人工条件下实现的这一过程，就是植物组织培养。 三、实验原理 植物细胞全能性的表达 脱分化（ dedifferentiation)：将来自已分化组织的已停止分裂的细胞从植物体部分的抑制性影响下解脱出来，恢复细胞的分裂活性。 再分化（ redifferentiation。